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primary mouse anti-notch3 ecd antibody clone 1e4  (Millipore)


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    Millipore primary mouse anti-notch3 ecd antibody clone 1e4
    Primary Mouse Anti Notch3 Ecd Antibody Clone 1e4, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti-notch3/anti+notch3/pmc12278194-37-14-20
    Average 90 stars, based on 1 article reviews
    primary mouse anti-notch3 ecd antibody clone 1e4 - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Incubation:

    Article Title: MiRNA-206 Affects the Recovery of Sciatic Function by Stimulating BDNF Activity through the Down-regulation of Notch3 Expression
    Article Snippet: The protein was then retransferred to PVDF membrane (Millipore, MA, USA, IPFL00010), and then closed with 5% skim milk and incubated with the following primary antibodies at 4°C: Mouse anti-Notch3 (1:1000, Millipore, MA, USA, APREST76106), Mouse anti-BNDF (1:1000, Sigma-Aldrich, USA, GF029) and Rabbit anti-GAPDH (1:1000, Millipore, MA, USA, G5262).

    Binding Assay:

    Article Title: MiRNA-206 Affects the Recovery of Sciatic Function by Stimulating BDNF Activity through the Down-regulation of Notch3 Expression
    Article Snippet: The protein was then retransferred to PVDF membrane (Millipore, MA, USA, IPFL00010), and then closed with 5% skim milk and incubated with the following primary antibodies at 4°C: Mouse anti-Notch3 (1:1000, Millipore, MA, USA, APREST76106), Mouse anti-BNDF (1:1000, Sigma-Aldrich, USA, GF029) and Rabbit anti-GAPDH (1:1000, Millipore, MA, USA, G5262).

    Luciferase:

    Article Title: MiRNA-206 Affects the Recovery of Sciatic Function by Stimulating BDNF Activity through the Down-regulation of Notch3 Expression
    Article Snippet: The protein was then retransferred to PVDF membrane (Millipore, MA, USA, IPFL00010), and then closed with 5% skim milk and incubated with the following primary antibodies at 4°C: Mouse anti-Notch3 (1:1000, Millipore, MA, USA, APREST76106), Mouse anti-BNDF (1:1000, Sigma-Aldrich, USA, GF029) and Rabbit anti-GAPDH (1:1000, Millipore, MA, USA, G5262).

    Reporter Gene Assay:

    Article Title: MiRNA-206 Affects the Recovery of Sciatic Function by Stimulating BDNF Activity through the Down-regulation of Notch3 Expression
    Article Snippet: The protein was then retransferred to PVDF membrane (Millipore, MA, USA, IPFL00010), and then closed with 5% skim milk and incubated with the following primary antibodies at 4°C: Mouse anti-Notch3 (1:1000, Millipore, MA, USA, APREST76106), Mouse anti-BNDF (1:1000, Sigma-Aldrich, USA, GF029) and Rabbit anti-GAPDH (1:1000, Millipore, MA, USA, G5262).

    Quantitative RT-PCR:

    Article Title: MiRNA-206 Affects the Recovery of Sciatic Function by Stimulating BDNF Activity through the Down-regulation of Notch3 Expression
    Article Snippet: The protein was then retransferred to PVDF membrane (Millipore, MA, USA, IPFL00010), and then closed with 5% skim milk and incubated with the following primary antibodies at 4°C: Mouse anti-Notch3 (1:1000, Millipore, MA, USA, APREST76106), Mouse anti-BNDF (1:1000, Sigma-Aldrich, USA, GF029) and Rabbit anti-GAPDH (1:1000, Millipore, MA, USA, G5262).

    Mutagenesis:

    Article Title: MiRNA-206 Affects the Recovery of Sciatic Function by Stimulating BDNF Activity through the Down-regulation of Notch3 Expression
    Article Snippet: The protein was then retransferred to PVDF membrane (Millipore, MA, USA, IPFL00010), and then closed with 5% skim milk and incubated with the following primary antibodies at 4°C: Mouse anti-Notch3 (1:1000, Millipore, MA, USA, APREST76106), Mouse anti-BNDF (1:1000, Sigma-Aldrich, USA, GF029) and Rabbit anti-GAPDH (1:1000, Millipore, MA, USA, G5262).

    Western Blot:

    Article Title: MiRNA-206 Affects the Recovery of Sciatic Function by Stimulating BDNF Activity through the Down-regulation of Notch3 Expression
    Article Snippet: The protein was then retransferred to PVDF membrane (Millipore, MA, USA, IPFL00010), and then closed with 5% skim milk and incubated with the following primary antibodies at 4°C: Mouse anti-Notch3 (1:1000, Millipore, MA, USA, APREST76106), Mouse anti-BNDF (1:1000, Sigma-Aldrich, USA, GF029) and Rabbit anti-GAPDH (1:1000, Millipore, MA, USA, G5262).

    Expressing:

    Article Title: MiRNA-206 Affects the Recovery of Sciatic Function by Stimulating BDNF Activity through the Down-regulation of Notch3 Expression
    Article Snippet: The protein was then retransferred to PVDF membrane (Millipore, MA, USA, IPFL00010), and then closed with 5% skim milk and incubated with the following primary antibodies at 4°C: Mouse anti-Notch3 (1:1000, Millipore, MA, USA, APREST76106), Mouse anti-BNDF (1:1000, Sigma-Aldrich, USA, GF029) and Rabbit anti-GAPDH (1:1000, Millipore, MA, USA, G5262).

    Over Expression:

    Article Title: MiRNA-206 Affects the Recovery of Sciatic Function by Stimulating BDNF Activity through the Down-regulation of Notch3 Expression
    Article Snippet: The protein was then retransferred to PVDF membrane (Millipore, MA, USA, IPFL00010), and then closed with 5% skim milk and incubated with the following primary antibodies at 4°C: Mouse anti-Notch3 (1:1000, Millipore, MA, USA, APREST76106), Mouse anti-BNDF (1:1000, Sigma-Aldrich, USA, GF029) and Rabbit anti-GAPDH (1:1000, Millipore, MA, USA, G5262).

    Activity Assay:

    Article Title: MiRNA-206 Affects the Recovery of Sciatic Function by Stimulating BDNF Activity through the Down-regulation of Notch3 Expression
    Article Snippet: The protein was then retransferred to PVDF membrane (Millipore, MA, USA, IPFL00010), and then closed with 5% skim milk and incubated with the following primary antibodies at 4°C: Mouse anti-Notch3 (1:1000, Millipore, MA, USA, APREST76106), Mouse anti-BNDF (1:1000, Sigma-Aldrich, USA, GF029) and Rabbit anti-GAPDH (1:1000, Millipore, MA, USA, G5262).



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    FIGURE 4 | Activation of RUNX1-CMYB-AQP4 signaling is downregulated <t>in</t> <t>NOTCH3-R170C</t> astrocyte. (A) Dual-luciferase reporter assays showing the effect of CMYB activation on Aqp4 transcription in HEK293T cells. Experiments were repeated for three times. *p < 0.05; by Student's t test (mean ± SEM). (B) Total or nucleus protein of NOTCH3-R170C and -WT astrocytes were subjected to western blot. Protein level was normalized to BACT or LAMIN B1 expression. Experiments were repeated for four times. *p < 0.05, **p < 0.01, and ***p < 0.001; by Student's t test (mean ± SEM). (C) Protein was extracted from NOTCH3-R170C or -WT astrocyte then subjected to immunoprecipitation (IP) with anti-NOTCH3 or anti-CMYB antibodies. IP products were analyzed with western blot to examine the interaction of NOTCH3 and RUNX1 or RUNX1 and CMYB, respectively. Experiments were repeated for 3 times. *p < 0.05; by Student's t test (mean ± SEM). (D) Immunostaining of CMYB (green) and RUNX1 (red) with NOTCH3-R170C or -WT astrocyte. (E, F) NOTCH3-R170C or -WT OSCs were labeled with RUNX1 (green) and S100B (red) (E) or CMYB (green) and S100B (red) (F), respectively. Percentage of RUNX1+ or CMYB+ cells among S100B+ astrocytes was calculated. Experiments were repeated for three times. *p < 0.05, **p < 0.01; by Student's t test (mean ± SEM). (G–H) Coronal brain sections of NOTCH3-R170C or -WT mice (16 weeks of age) were subjected to immunostaining of RUNX1 (red) and S100B (green) (G) or CMYB (red) and S100B (green) (H). N = 6 in each group. *p < 0.05, **p < 0.01; by Student's t test (mean ± SEM). (I) RUNX1 was overexpressed in NOTCH3-R170C or -WT astrocytes with lentiviral vectors carrying Runx1 cDNA (Runx1-OE) which were then subjected to western blot. Protein level was normalized to <t>GAPDH</t> expression. Experiments were repeated for four times. *p < 0.05, **p < 0.01; by one-way ANOVA (mean ± SEM).
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    Image Search Results


    FIGURE 4 | Activation of RUNX1-CMYB-AQP4 signaling is downregulated in NOTCH3-R170C astrocyte. (A) Dual-luciferase reporter assays showing the effect of CMYB activation on Aqp4 transcription in HEK293T cells. Experiments were repeated for three times. *p < 0.05; by Student's t test (mean ± SEM). (B) Total or nucleus protein of NOTCH3-R170C and -WT astrocytes were subjected to western blot. Protein level was normalized to BACT or LAMIN B1 expression. Experiments were repeated for four times. *p < 0.05, **p < 0.01, and ***p < 0.001; by Student's t test (mean ± SEM). (C) Protein was extracted from NOTCH3-R170C or -WT astrocyte then subjected to immunoprecipitation (IP) with anti-NOTCH3 or anti-CMYB antibodies. IP products were analyzed with western blot to examine the interaction of NOTCH3 and RUNX1 or RUNX1 and CMYB, respectively. Experiments were repeated for 3 times. *p < 0.05; by Student's t test (mean ± SEM). (D) Immunostaining of CMYB (green) and RUNX1 (red) with NOTCH3-R170C or -WT astrocyte. (E, F) NOTCH3-R170C or -WT OSCs were labeled with RUNX1 (green) and S100B (red) (E) or CMYB (green) and S100B (red) (F), respectively. Percentage of RUNX1+ or CMYB+ cells among S100B+ astrocytes was calculated. Experiments were repeated for three times. *p < 0.05, **p < 0.01; by Student's t test (mean ± SEM). (G–H) Coronal brain sections of NOTCH3-R170C or -WT mice (16 weeks of age) were subjected to immunostaining of RUNX1 (red) and S100B (green) (G) or CMYB (red) and S100B (green) (H). N = 6 in each group. *p < 0.05, **p < 0.01; by Student's t test (mean ± SEM). (I) RUNX1 was overexpressed in NOTCH3-R170C or -WT astrocytes with lentiviral vectors carrying Runx1 cDNA (Runx1-OE) which were then subjected to western blot. Protein level was normalized to GAPDH expression. Experiments were repeated for four times. *p < 0.05, **p < 0.01; by one-way ANOVA (mean ± SEM).

    Journal: CNS neuroscience & therapeutics

    Article Title: NOTCH3 Mutation Causes Glymphatic Impairment and Promotes Brain Senescence in CADASIL.

    doi: 10.1111/cns.70140

    Figure Lengend Snippet: FIGURE 4 | Activation of RUNX1-CMYB-AQP4 signaling is downregulated in NOTCH3-R170C astrocyte. (A) Dual-luciferase reporter assays showing the effect of CMYB activation on Aqp4 transcription in HEK293T cells. Experiments were repeated for three times. *p < 0.05; by Student's t test (mean ± SEM). (B) Total or nucleus protein of NOTCH3-R170C and -WT astrocytes were subjected to western blot. Protein level was normalized to BACT or LAMIN B1 expression. Experiments were repeated for four times. *p < 0.05, **p < 0.01, and ***p < 0.001; by Student's t test (mean ± SEM). (C) Protein was extracted from NOTCH3-R170C or -WT astrocyte then subjected to immunoprecipitation (IP) with anti-NOTCH3 or anti-CMYB antibodies. IP products were analyzed with western blot to examine the interaction of NOTCH3 and RUNX1 or RUNX1 and CMYB, respectively. Experiments were repeated for 3 times. *p < 0.05; by Student's t test (mean ± SEM). (D) Immunostaining of CMYB (green) and RUNX1 (red) with NOTCH3-R170C or -WT astrocyte. (E, F) NOTCH3-R170C or -WT OSCs were labeled with RUNX1 (green) and S100B (red) (E) or CMYB (green) and S100B (red) (F), respectively. Percentage of RUNX1+ or CMYB+ cells among S100B+ astrocytes was calculated. Experiments were repeated for three times. *p < 0.05, **p < 0.01; by Student's t test (mean ± SEM). (G–H) Coronal brain sections of NOTCH3-R170C or -WT mice (16 weeks of age) were subjected to immunostaining of RUNX1 (red) and S100B (green) (G) or CMYB (red) and S100B (green) (H). N = 6 in each group. *p < 0.05, **p < 0.01; by Student's t test (mean ± SEM). (I) RUNX1 was overexpressed in NOTCH3-R170C or -WT astrocytes with lentiviral vectors carrying Runx1 cDNA (Runx1-OE) which were then subjected to western blot. Protein level was normalized to GAPDH expression. Experiments were repeated for four times. *p < 0.05, **p < 0.01; by one-way ANOVA (mean ± SEM).

    Article Snippet: The following primary antibodies were used: rabbit anti- AQP4 (Proteintech 16,473- 1- AP, 1:1000), rabbit anti- RUNX1 (Affinity Biosciences AF6379, 1:1000), rabbit antiCMYB (Proteintech 17,800- 1- AP, 1:1000), rabbit anti- NOTCH3 (Proteintech 55,114- 1- AP, 1:1000), mouse anti- GAPDH (Proteintech 60,004- 1- Ig, 1:10000), mouse anti- β- ACTIN (Proteintech 66,009- 1- Ig, 1:10000), and mouse anti- LAMIN B1 (Proteintech 66,095- 1- Ig, 1:10000).

    Techniques: Activation Assay, Luciferase, Western Blot, Expressing, Immunoprecipitation, Immunostaining, Labeling